Packing AC Columns

The section describes a manual method

packing AC columns.

1. Pretreating Frits

Place the top and bottom frits into deionized water or 20% ethanol to remove the bubbles existing in the frit.

2. Loading Bottom Frit

Add a little deionized water or 20% ethanol to the bottom of a column. Then, push the bottom frit to the bottom by plunger and cap the column outlet.

3. Adding Resin

Transfer the required volume of resin by a plastic pipette to the column. Allow the resin to settle and fill the column.

4. Loading Top Frit

Push the top frit horizontally to the top of the column by plunger.

5. Sealing and Storage

Fill the column with an appropriate solvent. Take care and avoid the bubbles. Seal the upper cap and tighten the bottom cap.